Epigenetics is the study of chemical modifications that can alter phenotype without altering nucleotide sequence. One of the most well-characterised and widely studied modifications in mammalian — including human — genomes is 5mC DNA methylation, the addition of a methyl group to a cytosine nucleotide.

This getting started guide explores how researchers can sequence epigenetic modifications directly from native DNA molecules — without PCR or additional library preparation steps — to better understand how methylation influences phenotype and regulates gene expression.

In this guide, you will:

• Learn how direct DNA sequencing can support the analysis of epigenetic modifications

• Explore considerations for selecting the right tools and kits for your experimental goals

• Review real-world case studies demonstrating how scientists are examining aberrant methylation in gene promoters and its potential association with disease

Download your copy of this informative guide, today!