Single-cell RNA sequencing of the human transcriptome is a valuable research tool, shedding light on the complex mechanisms behind developmental processes, immune system functions, and diseases such as cancer. Full-length transcripts can be sequenced in single long nanopore reads, delivering unambiguous detection of isoforms and fusion transcripts that cannot be captured with short-read approaches. Additionally, nanopore reads enable SNV detection across complete transcripts for deep characterisation of clonal evolution and tumour heterogeneity.
This guide introduces the single-cell RNA nanopore sequencing workflow, for ultra-rich data without compromise.
In this getting started guide, you will:
- Find out about the benefits of Oxford Nanopore sequencing for single-cell transcriptomics, and how it reveals isoform-level information missed by legacy short-read technologies
- Read about the end-to-end single-cell nanopore sequencing workflow
- Discover how to go from 10x Genomics cell-barcoded cDNA samples to sequencing-ready libraries in around three hours
- Find out about the range of high-output PromethION sequencing devices
- Discover how single-cell nanopore sequencing data can be easily analysed using the EPI2ME workflow wf-single-cell
- Read case studies describing how researchers are utilising single-cell nanopore sequencing to enhance research into leukaemia and the mouse
Complete the form to the right to download your complimentary guide today!
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