Nanopore sequencing is the only available technology that directly reads native RNA transcripts, making it possible to quantify gene and isoform expression without PCR bias and detect RNA modifications. With long nanopore reads, isoforms can be resolved in full and poly-A tail lengths accurately estimated. This knowledge exchange described how direct RNA nanopore sequencing works and how it can enhance your research.
In this knowledge exchange, viewers learned:
In this knowledge exchange, viewers learned:
- How the Direct RNA Sequencing Kit enables the preparation and nanopore sequencing of native RNA, including the detection of RNA modifications.
- About the direct RNA sequencing workflow, including how to prepare libraries with the Direct RNA Sequencing Kit, which sequencing device to choose to meet experimental goals, and how to analyse data.
- What direct RNA nanopore sequencing can bring to research across numerous applications, with a worked example.
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